Innovative connections for steel-concrete-trussed beams: a patented solution

The most recent design strategies welcome the adoption of innovative techniques for seismic energy input mitigation, aiming to achieve high dissipation capacity, prevent the structure from collapse and ensure the serviceability of the construction. Friction damper devices have been widely adopted in framed steel structures for decades, while their introduction in different structural types is still under investigation. This paper presents the outcomes of innovative research supported by the industry and conducted on beam-to-column connections of RC structures in which the beams are Hybrid Steel-Trussed Concrete Beams (HSTCBs) and the columns are classical RC pillars. An innovative solution, recently patented, has been found for the mitigation of the effects of seismic cyclic actions on small-sized beam-column joints, typically characterised by a large amount of longitudinal reinforcement due to the small effective depth of the beam. This paper collects the main featuring steps of the innovative research, which has led to the patented solution. The calculation procedure for designing the proposed connection is shown, and the validation through 3D finite element modelling is described. For the structural analysis of the joint, several monotonic and cyclic simulations have been carried out with the scope of investigating different design moment values. The finite element results proved that the patented solution is effective in preventing beam, column and joint from damage and it is suitable for exhibiting adequate dissipative capacity ensured by a flexural behaviour dominated by wide and stable hysteresis loops

Experimental characterization of friction properties of materials for innovative beam-to-column dissipative connection for low-damage RC structures

Low-damage design of structures in seismic-prone areas is becoming an efficient strategy to obtain "earthquake-proof" buildings, i.e. buildings that, even in the case of severe seismic actions, experience a low or negligible amount of damage. Besides the safeguard of human lives, this design strategy aims also to limit the downtime of buildings, which represents a significant source of economic loss, and to ensure an immediate occupancy in the aftermath of an earthquake. In this context, focusing on moment-resisting frames (MRFs), several solutions have been developed for the beam-to-column connections (BCCs) of steel and precast/prestressed concrete structures, but very few for cast-in-situ reinforced concrete (RC) structures. This paper focuses on a recently-proposed friction-based BCC for MRFs made with hybrid steel-trussed concrete beams (HSTCBs). The latter are made by a spatial lattice built using V-shaped rebars and a steel bottom plate, which eases the introduction of a friction dissipative device. HSTCBs are usually characterized by a small effective depth, which leads to a large amount of longitudinal rebars. The latter, together with a small-sized beam-column joint, make it potentially subjected to severe damage, which reduces its dissipative capacity. The shear force acting on the joint can be reduced by endowing the BCC with a friction device, with the aim of increasing the lever arm of the bending moment transferred between beam and joint, preventing the latter from damage. To evaluate the mechanical performance of the above connection, two experimental programs have been carried out at the Structures Laboratory of the University of Palermo. The first one focused on the characterization of the friction properties of two different materials (thermal sprayed aluminum and brass), by means of a linear dissipative device subjected to cyclic load. The second one tested a beam-to-column subassembly endowed with the recently-proposed connection in which the dissipative device was made with the best performing friction material tested before. The results of the cyclic tests are presented and commented, showing the promising performance of such connection in providing a low-damage behavior and a satisfactory dissipative capacity

Seismic Performance of Earthquake-Resilient RC Frames Made with HSTC Beams and Friction Damper Devices

Seismic behavior of RC frames with hybrid steel-trussed concrete beams is affected by panel zone damage due to a large amount of longitudinal reinforcement. Here the seismic efficiency of innovative frames characterized by friction damper devices (FDDs) at beam-to-column connections is compared against traditional frame. Three configurations are investigated: FDDs alone; FDDs with column-to-foundation connections having preloaded threaded bars and disk springs; FDDs with self-centering friction devices. Non-linear analyses show that FDDs alone prevent plastic hinge formation at beam ends and beam–column joint damage. FDDs with self-centering friction devices effectively limit both peak and residual drifts, avoiding column base plasticization

Low-Damage Friction Connections in Hybrid Joints of Frames of Reinforced-Concrete Buildings

Seismic-resilient buildings are increasingly designed following low-damage and free-from-damage design strategies that aim to protect the structure’s primary load-bearing systems under ultimate-level seismic loads. With this scope, damping devices are located in accessible and easy-to-inspect sites within the main structural frames where the damage concentrates, allowing the primary structure to remain mostly undamaged or easily repairable after a severe earthquake. This paper analyses the effects of friction-damping devices in structural joints of RC buildings endowed with hybrid steel-trussed concrete beams (HSTCBs) and standard RC columns. The study proposes innovative solutions to be adopted into RC moment-resisting frames (MRFs) at beam-to-column connections (BCCs) and column-base connections (CBCs). The cyclic behaviour of the joint is analysed through 3D finite element models, while pushover and non-linear time history analyses are performed on simple two-storey and two-span MRFs endowed with the proposed devices. The main results show that the BCC endowed with curved slotted holes and Perfobond connectors is the most effective in preventing the damage that might occur in beam, column, and joint, and it is adequate to guarantee good dissipative properties. For CBCs, the results showed that the re-centering system with friction pads is the most effective in containing the peak and residual drifts, preventing the plasticization of the column base

Reversible effect of magnetic fields on human lymphocyte activation patterns: different sensitivity of naive and memory lymphocyte subsets.

The aim of this study was to investigate the influence of 50 Hz magnetic or static magnetic fields of 0.5 mT on subsets of human CD4+ T cells in terms of cytokine release/content, cell proliferation and intracellular free calcium concentration. CD4+ T cells can be divided into different subsets on the basis of surface marker expression, such as CD45, and T cells can be divided into naive (CD45RA+) and memory (CD45RA2) cells. In this study, the effects of magnetic fields after 24 and 48 h of cell culture were analyzed. We found that the CD4+CD45RA2 T subset were more sensitive after 2 h of exposure. Decreases in the release/content of IFN-c, in cell proliferation and in intracellular free calcium concentrations were observed in exposed CD4+CD45RA2 T cells compared to CD4+CD45RA+ T cells. The results suggest that exposure to the magnetic fields induces a delay in the response to stimulants and that modifications are rapidly reversible, at least after a short exposure

The role of copper (Ii) on kininogen binding to tropomyosin in the presence of a histidine–proline-rich peptide

The antiangiogenic activity of the H/P domain of histidine–proline-rich glycoprotein is mediated by its binding with tropomyosin, a protein exposed on endothelial cell-surface during the angiogenic switch, in presence of zinc ions. Although it is known that copper ion serum concentration is significantly increased in cancer patients, its role in the interaction of H/P domain with tropomyosin, has not yet been studied. In this paper, by using ELISA assay, we determined the modulating effect of TetraHPRG peptide, a sequence of 20 aa belonging to H/P domain, on the binding of Kininogen (HKa) with tropomyosin, both in absence and presence of copper and zinc ions. A potentiometric study was carried out to characterize the binding mode adopted by metal ions with TetraHPRG, showing the formation of complex species involving imidazole amide nitrogen atoms in metal binding. Moreover, circular dichroism showed a conformational modification of ternary systems formed by TetraHPRG, HKa and copper or zinc. Interestingly, slight pH variation influenced the HKa-TetraHPRG-tropomyosin binding. All these results indicate that both metal ions are crucial in the interaction between TetraHPRG, tropomyosin and HKa

Acute bleeding obstruction pancreatitis after Roux-en-Y anastomosis in total gastrectomy: a single center experience

Anastomotic intraluminal bleeding is a well-known complication after total gastrectomy. Nevertheless, few data are published on acute bleeding obstruction pancreatitis (BOP) due to a bleeding from the jejunojejunostomy (JJ). In this paper we describe our experience. A total of 140 gastrectomies for EGJ cancer were performed in our Institute from January 2012 to January 2017. All reconstructions were performed with a Roux-en-Y anastomosis: a mechanical end-to-side esophago-jejunostomy and a mechanical end-to-side JJ. Three patients suffered from a bleeding at the JJ with a consequent BOP. We analyzed the time of diagnosis, the treatment and the outcomes. The three patients presented anemia at the laboratory findings on postoperative day (POD) 1. In patient I laboratory findings of acute pancreatitis were found in POD 2. CT scan was performed and showed signs of BOP. Endoscopic treatment was tried without success. Therefore, patient underwent surgery: JJ take down, bleeding control and anastomosis rebuild were performed. In spite of this the patient died of MOF in POD 4. Patient II had a persistent anemia treated with blood transfusions until POD 3, when laboratory tests showed increased lipase and bilirubin levels. Patient was successfully treated with endoscopy but several blood transfusions and a prolonged recovery were necessary. Patient III had laboratory findings of acute pancreatitis on POD 1. Immediate surgery was performed and patient was discharged on POD 9 without sequelae. BOP is a rare but deadly complication after Roux-en-Y anastomosis. An early diagnosis and an aggressive treatment seem to improve the outcome

Experimental Investigation on Innovative Stress Sensors for Existing Masonry Structures Monitoring

Historical masonry structures often suffer gradual deterioration that in many cases can compromise the safety levels and the operating conditions of the buildings. In this context, Structural Health Monitoring (SHM) is an effective tool for the prediction of the structural behaviour and the state of conservation of buildings. Although many monitoring systems have recently been proposed, there is a lack of practical application of low-cost systems. This paper presents an experimental study based on the use of two innovative stress sensors—capacitive stress sensor and ceramic stress sensor—for the monitoring of existing masonry elements. In order to reproduce the actual conditions of onsite masonry, sensors are post-installed in the mortar joints of two series of pre-stressed specimens made of calcarenite stone masonry and clay brick masonry. The best practice of post-installation of the two sensors is investigated. The reliability of the proposed sensors is evaluated through comparison with data recorded from classical measurement devices

Hyaluronan-carnosine conjugates inhibit Aβ aggregation and toxicity

Alzheimer’s disease is the most common neurodegenerative disorder. Finding a pharmacological approach that cures and/or prevents the onset of this devastating disease represents an important challenge for researchers. According to the amyloid cascade hypothesis, increases in extracellular amyloid-β (Aβ) levels give rise to different aggregated species, such as protofibrils, fibrils and oligomers, with oligomers being the more toxic species for cells. Many efforts have recently been focused on multi-target ligands to address the multiple events that occur concurrently with toxic aggregation at the onset of the disease. Moreover, investigating the effect of endogenous compounds or a combination thereof is a promising approach to prevent the side effects of entirely synthetic drugs. In this work, we report the synthesis, structural characterization and Aβ antiaggregant ability of new derivatives of hyaluronic acid (Hy, 200 and 700 kDa) functionalized with carnosine (Car), a multi-functional natural dipeptide. The bioactive substances (HyCar) inhibit the formation of amyloid-type aggregates of Aβ42 more than the parent compounds; this effect is proportional to Car loading. Furthermore, the HyCar derivatives are able to dissolve the amyloid fibrils and to reduce Aβ-induced toxicity in vitro. The enzymatic degradation of Aβ is also affected by the interaction with HyCar

Distinctive features of tumor-infiltrating gd T lymphocytes in human colorectal cancer

gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4%gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage